Based on the data of China rural Development Survey (CRDS) from 2007 to 2015, this study uses the ordered Logit with fixed effect model to study the influence of public investment preference difference between village cadres and villagers on villagers’ satisfaction level towards rural public service from the gender perspective. The results show that the preference differences between village cadres and villagers have a negative impact on villagers’ satisfaction level with public services, which is most prominent in road and school public services. Systematic differences of preference difference and satisfaction level towards different types of rural public goods could be identified between male and female, which leads to their different focus on public services: men pay more attention to road construction, while women to school construction. This paper further enriches the literature on information asymmetry in local governance in China. At the same time, paying attention to the topic, how preference difference between village cadres and villagers would leave an impact on villagers’ satisfaction with public services from a gender perspective, could help to improve the supply efficiency and inclusiveness of rural public goods, and finally, improve the well-being of villagers.